Optimized RNA Extraction and Northern Hybridization in Streptomycetes

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

development and implementation of an optimized control strategy for induction machine in an electric vehicle

in the area of automotive engineering there is a tendency to more electrification of power train. in this work control of an induction machine for the application of electric vehicle is investigated. through the changing operating point of the machine, adapting the rotor magnetization current seems to be useful to increase the machines efficiency. in the literature there are many approaches wh...

Magnetic nanoparticles: a promising component in RNA extraction process

Magnetic nanoparticles separation technology is a method for quick and easy extraction biomolecules such as proteins, DNA and RNA. The present work describes total RNA isolation procedure from transformed rose petals in our laboratory using magnetic nanoparticles as a solid phase absorbant. Petals are the main sources of secondary metabolites, i.e. carotenoids, anthocyanins, flavonoids and phen...

Extraction and comparison of gene expression patterns from 2D RNA in situ hybridization images

MOTIVATION Recent advancements in high-throughput imaging have created new large datasets with tens of thousands of gene expression images. Methods for capturing these spatial and/or temporal expression patterns include in situ hybridization or fluorescent reporter constructs or tags, and results are still frequently assessed by subjective qualitative comparisons. In order to deal with availabl...

Northern hybridization: rapid and simple electrophoretic conditions.

Current methods for Northern blot analysis to determine mRNA abundance in tissues are tedious and utilize toxic chemicals. The requirement for chemicals of high purity, free of ribonucleases, and the lability of RNA, in comparison to DNA, are additional factors preventing wider use of the technique. For Northern blot analysis, RNA is generally denatured with glyoxal (1) or formaldehyde (2) prio...